Antifungal activity on the strain of lasiodiplodia. Identification of fungus b4d was also done through molecular characterization using 18s internal transcribed spacer its regions of ribosomal deoxyribonucleic acid rdna. Direct microscopy analysis of the bronchoalveolar lavage specimens showed septate hyphae. The causal fungus was identified as lasiodiplodia pseudotheobromae based on morphology and analysis of the internal transcribed spacer its and translation elongation factor 1alpha ef1. Lasiodiplodia theobromae is one of the most frequent fungal pathogens associated with dieback, gummosis, leaf spot, stem. A multiomics analysis of the grapevine pathogen lasiodiplodia. The pathogenicity of lasiodiplodia theobromae and fusarium proliferatum separate and together was confirmed in inoculations of teak elite genotypes, fulfilling kochs postulates. Metabolites of lasiodiplodia theobromae journal of the. Lasiodiplodia theobromae, neofusicoccum parvum, nectria pseudotrichia, and fusarium solani, was determined using thedualculturetechnique8. Lasiodiplodia theobromae was also isolated from wood samples saw dust which used as raw materials for spawn packets preparation to grow commercial mushroom at national mushroom development and extension centre, savar. Download fulltext pdf download fulltext pdf read fulltext. Pdf characterization of lasiodiplodia theobromae causing. Jan 01, 2016 lasiodiplodia theobromae was successfully reisolated from the lesions, but not from the negative control wounds. Polymerase chain reaction amplified a band with a sequence that was 100% homologous with lasiodiplodia theobromae.
Lasiodiplodia theobromae phyorhoverview eppo global database. Phylogenetic analyses identified three wellsupported clades within this group of isolates, one. Aladawi ao, deadman ml, alrawahi ak, khan aj, almaqbali ym. Nov 30, 2019 blue staining on rubberwood hevea brasiliensis is a common kind of defect.
The aim of this study was to characterise the symptoms of dieback of table grapes. In vitro inhibition of botryosphaeria dothidea and lasiodiplodia. Lasiodiplodia, commonly referred to as blacksoot disease, is a. Exploitation of medicinal plants to combat the growth of l. Characterization of lasiodiplodia pseudotheobromae associated with citrus stemend rot disease in bangladesh. Antifungal activity of propyl disulphide from neem against. Oct 23, 2014 production of table grapes is a major export commodity in the region of piura, one of the most important grapeproduction areas of peru. Mar 19, 2008 lasiodiplodia theobromae is a common, widespread pathogen of tropical woody trees, causing shoot blight and dieback of trees and shrubs and blue stain in timber mohali et al. The latter two cultivars served as checks for susceptibility and resistance respectively based on their reaction to rice blast. A dematiaceous mold was recovered and identified as l. Genetic diversity and population structure of lasiodiplodia.
Lasiodiplodia theobromae was successfully reisolated from the lesions, but not from the negative control wounds. We report a patient with fungal keratitis caused by l. Feb 01, 2020 lasiodiplodia theobromae is a colorchanging fungus widely found in wood, such as rubber wood, masson pine, white birch, and poplar 25,26, which secretes melanin to stain cell walls, showing bluegray or blueblack discoloration on the surface of wood, but the pigments generally penetrate into the surface of wood and are confined to sapwood. Microbial dyeinginfection behavior and influence of. The fungus was first reported on cocoa in cameroon in 1895, and has since caused minor symptoms of charcoal pod rot. Pdf the fungal pathogen lasiodiplodia theobromae has been associated with numerous plant diseases on a wide.
It causes rotting and dieback in most species it infects. It was microscopically identified on the basis of cultural and morphological characters. The symptoms were chlorosis and later necrosis of leaves, mostly those second or third from the stem tips. Diversity and host association of the tropical tree. It also infects biancaea sappan, a species of flowering tree also known as sappanwood on rare occasions it has been found to cause fungal. First report of lasiodiplodia theobromae and fusarium. Samples of theobroma cacao seedlings and mature trees with vascular streak dieback vsdlike symptoms were observed in davao, philippines in 2014. Lasiodiplodia theobromae is a common pathogen that occurs on a large number of hosts in the tropics and. Successful treatment of lasiodiplodia theobromae keratitis. Dothideomycetes, botryosphaeriaceae is the accepted name of the species treated in this paper, prevailing over both the basionym botryodiplodia theobromae pat. Lasiodiplodia theobromae pakistan journal of botany. Characteristics of lasiodiplodia theobromae from rosa rugosa. Dieback due to lasiodiplodia theobromae, a new constraint.
Curtis arx, after the introduction in mycology of the principle one speciesone name. Physical and mechanical properties of rubberwood hevea. Lasiodiplodia, commonly referred to as blacksoot disease, is a significant pathogen in tropical forestry. Characterization of lasiodiplodia pseudotheobromae. May 26, 2020 postharvest pathogens were isolated and identified as lasiodiplodia theobromae and neofusicoccum parvum causing the stem end rot of mango fruit. There currently exists much research focused on the prevention and control of blue staining. Pdf pathogenicity of lasiodiplodia theobromae and fusarium. Lasiodiplodia theobromae and diplodia seriata, were frequently isolated. Sterilepdawaspouredinto petri dishes 9cm in diameter. Coconut leaf blight pathogen lasiodiplodia theobromae pat. Dieback due to lasiodiplodia theobromae, a new constraint to. Plant diseases attributed to botryodiplodia theobromae pat. Virulence tests of neofusicoccum parvum, lasiodiplodia. Lasiodiplodia theobromae is a plant pathogen with a very wide host range.
Characterization of lasiodiplodia pseudotheobromae associated. Presentation mode open print download current view. Lasiodiplodia theobromae causing canker and shoot blight a total of nine isolates of lasiodiplodia of ig in turkey. This is the first report of root rot and collar rot disease of physic nut in india. Prajapati hn, patel jk, patil rk 2014 lasiodiplodia theobromae. Lasiodiplodia theobromae causes vascular streak dieback vsd. A plantgrowth inhibitor produced by lasiodiplodia theobromae has been shown to be jasmonic acid ia. Phylogeny, identification, and pathogenicity of lasiodiplodia.
The causal agent of root rot and collar rot of biofuel plant jatropha curcas and its variability h. Antifungal susceptibility, morphological and molecular. Fruit rot disease of postharvest longan is a major limiting factor for the longan market in thailand. Lasiodiplodia theobromae is a common pathogen that occurs on a large number of hosts in the tropics and subtropics. Field applications were made by spraying or painting after lesionscraping. Lasiodiplodia theobromae an overview sciencedirect topics. This paper offers an overview of the endophytic occurrence of lasiodiplodia theobromae dothideomycetes, botryosphaeriaceae, a species. Lasiodiplodia is a genus of fungi in the family botryosphaeriaceae. For example the conidial size of lasiodiplodia theobromae was 19.
Inoculation was carried out on 6 month old teaks clones, by placing 1. We do not use these to store personal information about you. Blast result indicated that the fungus was 100% similar to that of l. Documents about lasiodiplodia theobromae phyorh this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Diplodia theobromae associated with sudden decline of mango in the sultanate of oman. Pdf morphological and molecular data reveal cryptic. Identification and pathogenicity of lasiodiplodia theobromae and diplodia seriata, the causal agents of bot canker disease of grapevines in mexico. Lasiodiplodia pseudotheobromae causes postharvest fruit. Pdf lasiodiplodia species associated with dieback disease. Lasiodiplodia theobromae pneumonia in a liver transplant. Studies on the isolation of seedborne fungi on kenaf seed have revealed that l. Evaluation of bioagents to control lasiodiplodia theobromae pat. Lasiodiplodia pseudotheobromae causes postharvest fruit rot. Botryodiplodia theobromae is a causative fungus of this disease khanzada et al.
Diversity and host association of the tropical tree endophyte. Six other new fungal metabolites have been isolated from l. The research conveyed in this paper primarily used lasiodiplodia theobromae to dye rubberwood, and used scanning electron. Sep 11, 2019 lasiodiplodia theobromae botryosphaeriaceae, ascomycota is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. Full length article morphological, pathogenic and molecular. Sep 24, 2017 samples of theobroma cacao seedlings and mature trees with vascular streak dieback vsdlike symptoms were observed in davao, philippines in 2014. In 1971, jasmonic acid was isolated from the culture filtrate of a fungus of botryodiplodia theobromae lasiodiplodia theobromae as a plant growth inhibitor and its chemical structure elucidated. First report of lasiodiplodia theobromae causing spadix. In vitro percent of inhibition of lasiodiplodia theobromae by four trichoderma spp. Lasiodiplodia theobromae causes vascular streak dieback.
Morphological and molecular data reveal cryptic speciation. First report of lasiodiplodia theobromae on macadamia. Lasiodiplodia theobromae diplodia pod rot of cocoa cabi. Antifungal activity of propyl disulphide at different concentrations was determined in comparison with ethanol and distilled water. Characteristics of lasiodiplodia theobromae from rosa. Antifungal activity on the strain of lasiodiplodia theobromae. Lasiodiplodia theobromae phyorhoverview eppo global. Infection routes were determined by inoculating unwounded stems and.
However, little research has been concentrated on the utilization of blue staining for green dyeing. His condition was complicated by enterococcus faecium peritonitis. Morphological and molecular data reveal cryptic speciation in. Potential for biocontrol of lasiodiplodia theobromae pat. Lasiodiplodia theobromae affect the cultivation of palto persea americana under the conditions of piura, peru abstract the purposes of the present research were to describe the symptomatology, to study the incidence and. Lasiodiplodia theobromae in the root rot disease complex. Universiti putra malaysia characterization of lasiodiplodia. Dec 20, 2019 the pathogen was identified as lasiodiplodia theobromae, based on morphology and the nucleotide sequences of the internal transcribed spacer its and translation elongation factor 1 ef1 genes. To extract, identify and evaluate in vitro the antifungal activity of the phytochemical groups of ageratum conyzoides and newbouldia laevis on the strain of lasiodiplodia theobromae. Lasiodiplodia theobromae is a plurivorous pathogen of tropical and subtropical woody and fruit trees. Continuing to use this website means you agree to our use of cookies. Crown rot disease occurs in banana and is caused by complex of lasiodiplodia theobromae, colletotrichum musae and fusarium spp. Pdf mango plantations in different areas of sindh were found to suffer from a decline disease showing symptoms of drying of branches from the tip. Crown rot disease symptoms first appear on the cut surface of the crescentshaped crown, and the infected tissues become black and soft.
Jan 30, 2018 lasiodiplodia theobromae is a rare ocular pathogen. Lasiodiplodia theobromae phyorhdocuments eppo global. Characteristic symptoms of botryosphaeria dieback were observed in vineyards throughout this region. Lasiodiplodia theobromae botryosphaeriaceae, ascomycota is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. It can be controlled effectively by demethylation inhibitor dmi fungicides, but the occurrence of dmi resistance may decrease fungicide efficacy. Characterization of lasiodiplodia theobromae and l. Pdf morphological and molecular data reveal cryptic species. Lasiodiplodia theobromae is a seedborne fungal pathogen that infects a variety of crop seeds. Pathogenicity test proved kochs postulates, supporting that l. College of agriculture, anand agricultural university, anand 388 110 email. Neofusicoccum parvum is a recently reported pathogen affecting theobroma cacao l. In this study, the variation in the genetic diversity of 117 l. Identification and pathogenicity of lasiodiplodia theobromae. Materials and methods lasiodiplodia theobromae was isolated from inflorescences of cashew showing typical symptoms of inflorescence blight disease in ibadan, nigeria.
Determining infection routes and virulence are essential for assessing the impact of n. Many fungi reported for endophytic occurrence are better known as plant pathogens on different crops, raising questions about their actual relationships with the hosts and other plants in the biocoenosis and about the factors underlying the lifestyle shift. Lasiodiplodia theobromae as a plant growth inhibitor and its chemical structure elucidated. The fungus lasiodiplodia theobromae is the only species that has been reported to be. It also infects biancaea sappan, a species of flowering tree also known as sappanwood. Evaluation of nerica rice lines for their reaction to lasiodiplodia theobromae nine nerica 1,2,4,5,7,8,10,11,14 with wab 56104 and os6 were obtained from the africa rice centre. Lasiodiplodia theobromae telemorph botryosphaeria rhodina, a member of the botryosphaeriaceae, has been recognized as an. Bar graph showing the average lesion length mm resulting from two experiments in which branches of rosa rugosa were inoculated with lasiodiplodia theobromae. It can be controlled effectively by demethylation inhibitor dmi fungicides, but occurrence of dmi resistance may decrease fungicide efficacy. Histopathology analysis revealed the presence fungi invading descemets membrane of the cornea. The patient was a 75yearold male, a farmer with diabetes type ii, and no previous history of ocular trauma.
Our previous studies have established that lasiodiplodia theobromae syn. Dieback is one of the most common diseases of mango in different parts of the world, including oman. Xie hh, wei jg, liu f, pan xh, yang xb 2014 first report of mulberry root rot caused by lasiodiplodia theobromae in china. Stemend rot is the major postharvest disease of coconut in. Despite efforts to control the disease using different. The fungus was characterized by septate, highly bulged fungal.
Lasiodiplodia theobromaekeratitisvoriconazolefungus. It is a common post harvest fungus disease of citrus known as stemend rot. Pdf identification and pathogenicity of lasiodiplodia. Vista do control of the fungi lasiodiplodia theobromae, the causal. Iieffect of different concentration of bavistin and diathane m45 on mycelia growth of l. Lasiodiplodia theobromae is a plant pathogenic fungus from the family botryosphaeriaceae that is commonly found in tropical.
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